Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Identifieur interne : 000340 ( 2020/Analysis ); précédent : 000339; suivant : 000341Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Auteurs : Giwan Seo [Corée du Sud] ; Geonhee Lee [Corée du Sud] ; Mi Jeong Kim [Corée du Sud] ; Seung-Hwa Baek [Corée du Sud] ; Minsuk Choi [Corée du Sud] ; Keun Bon Ku [Corée du Sud] ; Chang-Seop Lee [Corée du Sud] ; Sangmi Jun [Corée du Sud] ; Daeui Park [Corée du Sud] ; Hong Gi Kim [Corée du Sud] ; Seong-Jun Kim [Corée du Sud] ; Jeong-O Lee [Corée du Sud] ; Bum Tae Kim [Corée du Sud] ; Edmond Changkyun Park [Corée du Sud] ; Seung Il Kim [Corée du Sud]Source :
- ACS nano [ 1936-086X ] ; 2020.
Abstract
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.
DOI: 10.1021/acsnano.0c02823
PubMed: 32293168
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000122
- to stream PubMed, to step Curation: 000122
- to stream PubMed, to step Checkpoint: 000277
- to stream Ncbi, to step Merge: 003A69
- to stream Ncbi, to step Curation: 003A69
- to stream Ncbi, to step Checkpoint: 003A69
- to stream Main, to step Merge: 000340
- to stream Main, to step Curation: 000340
- to stream Main, to step Exploration: 000340
- to stream 2020, to step Extraction: 000340
Links to Exploration step
pubmed:32293168Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.</title><author><name sortKey="Seo, Giwan" sort="Seo, Giwan" uniqKey="Seo G" first="Giwan" last="Seo">Giwan Seo</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Geonhee" sort="Lee, Geonhee" uniqKey="Lee G" first="Geonhee" last="Lee">Geonhee Lee</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Mi Jeong" sort="Kim, Mi Jeong" uniqKey="Kim M" first="Mi Jeong" last="Kim">Mi Jeong Kim</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author><author><name sortKey="Baek, Seung Hwa" sort="Baek, Seung Hwa" uniqKey="Baek S" first="Seung-Hwa" last="Baek">Seung-Hwa Baek</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Minsuk" sort="Choi, Minsuk" uniqKey="Choi M" first="Minsuk" last="Choi">Minsuk Choi</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Ku, Keun Bon" sort="Ku, Keun Bon" uniqKey="Ku K" first="Keun Bon" last="Ku">Keun Bon Ku</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Chang Seop" sort="Lee, Chang Seop" uniqKey="Lee C" first="Chang-Seop" last="Lee">Chang-Seop Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986</wicri:regionArea><wicri:noRegion>Jeonju 54986</wicri:noRegion></affiliation></author><author><name sortKey="Jun, Sangmi" sort="Jun, Sangmi" uniqKey="Jun S" first="Sangmi" last="Jun">Sangmi Jun</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Park, Daeui" sort="Park, Daeui" uniqKey="Park D" first="Daeui" last="Park">Daeui Park</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hong Gi" sort="Kim, Hong Gi" uniqKey="Kim H" first="Hong Gi" last="Kim">Hong Gi Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Jeong O" sort="Lee, Jeong O" uniqKey="Lee J" first="Jeong-O" last="Lee">Jeong-O Lee</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Bum Tae" sort="Kim, Bum Tae" uniqKey="Kim B" first="Bum Tae" last="Kim">Bum Tae Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Park, Edmond Changkyun" sort="Park, Edmond Changkyun" uniqKey="Park E" first="Edmond Changkyun" last="Park">Edmond Changkyun Park</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seung Il" sort="Kim, Seung Il" uniqKey="Kim S" first="Seung Il" last="Kim">Seung Il Kim</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32293168</idno><idno type="pmid">32293168</idno><idno type="doi">10.1021/acsnano.0c02823</idno><idno type="wicri:Area/PubMed/Corpus">000122</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000122</idno><idno type="wicri:Area/PubMed/Curation">000122</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000122</idno><idno type="wicri:Area/PubMed/Checkpoint">000277</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000277</idno><idno type="wicri:Area/Ncbi/Merge">003A69</idno><idno type="wicri:Area/Ncbi/Curation">003A69</idno><idno type="wicri:Area/Ncbi/Checkpoint">003A69</idno><idno type="wicri:Area/Main/Merge">000340</idno><idno type="wicri:Area/Main/Curation">000340</idno><idno type="wicri:Area/Main/Exploration">000340</idno><idno type="wicri:Area/2020/Extraction">000340</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.</title><author><name sortKey="Seo, Giwan" sort="Seo, Giwan" uniqKey="Seo G" first="Giwan" last="Seo">Giwan Seo</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Geonhee" sort="Lee, Geonhee" uniqKey="Lee G" first="Geonhee" last="Lee">Geonhee Lee</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Mi Jeong" sort="Kim, Mi Jeong" uniqKey="Kim M" first="Mi Jeong" last="Kim">Mi Jeong Kim</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author><author><name sortKey="Baek, Seung Hwa" sort="Baek, Seung Hwa" uniqKey="Baek S" first="Seung-Hwa" last="Baek">Seung-Hwa Baek</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Minsuk" sort="Choi, Minsuk" uniqKey="Choi M" first="Minsuk" last="Choi">Minsuk Choi</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Ku, Keun Bon" sort="Ku, Keun Bon" uniqKey="Ku K" first="Keun Bon" last="Ku">Keun Bon Ku</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Chang Seop" sort="Lee, Chang Seop" uniqKey="Lee C" first="Chang-Seop" last="Lee">Chang-Seop Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju 54986</wicri:regionArea><wicri:noRegion>Jeonju 54986</wicri:noRegion></affiliation></author><author><name sortKey="Jun, Sangmi" sort="Jun, Sangmi" uniqKey="Jun S" first="Sangmi" last="Jun">Sangmi Jun</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Park, Daeui" sort="Park, Daeui" uniqKey="Park D" first="Daeui" last="Park">Daeui Park</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Hong Gi" sort="Kim, Hong Gi" uniqKey="Kim H" first="Hong Gi" last="Kim">Hong Gi Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Jeong O" sort="Lee, Jeong O" uniqKey="Lee J" first="Jeong-O" last="Lee">Jeong-O Lee</name><affiliation wicri:level="1"><nlm:affiliation>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Bum Tae" sort="Kim, Bum Tae" uniqKey="Kim B" first="Bum Tae" last="Kim">Bum Tae Kim</name><affiliation wicri:level="1"><nlm:affiliation>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114</wicri:regionArea><wicri:noRegion>Daejeon 34114</wicri:noRegion></affiliation></author><author><name sortKey="Park, Edmond Changkyun" sort="Park, Edmond Changkyun" uniqKey="Park E" first="Edmond Changkyun" last="Park">Edmond Changkyun Park</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seung Il" sort="Kim, Seung Il" uniqKey="Kim S" first="Seung Il" last="Kim">Seung Il Kim</name><affiliation wicri:level="1"><nlm:affiliation>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119</wicri:regionArea><wicri:noRegion>Cheongju 28119</wicri:noRegion></affiliation></author></analytic><series><title level="j">ACS nano</title><idno type="eISSN">1936-086X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 10<sup>1</sup> pfu/mL) and clinical samples (LOD: 2.42 × 10<sup>2</sup> copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Seo, Giwan" sort="Seo, Giwan" uniqKey="Seo G" first="Giwan" last="Seo">Giwan Seo</name></noRegion><name sortKey="Baek, Seung Hwa" sort="Baek, Seung Hwa" uniqKey="Baek S" first="Seung-Hwa" last="Baek">Seung-Hwa Baek</name><name sortKey="Choi, Minsuk" sort="Choi, Minsuk" uniqKey="Choi M" first="Minsuk" last="Choi">Minsuk Choi</name><name sortKey="Jun, Sangmi" sort="Jun, Sangmi" uniqKey="Jun S" first="Sangmi" last="Jun">Sangmi Jun</name><name sortKey="Kim, Bum Tae" sort="Kim, Bum Tae" uniqKey="Kim B" first="Bum Tae" last="Kim">Bum Tae Kim</name><name sortKey="Kim, Hong Gi" sort="Kim, Hong Gi" uniqKey="Kim H" first="Hong Gi" last="Kim">Hong Gi Kim</name><name sortKey="Kim, Mi Jeong" sort="Kim, Mi Jeong" uniqKey="Kim M" first="Mi Jeong" last="Kim">Mi Jeong Kim</name><name sortKey="Kim, Seong Jun" sort="Kim, Seong Jun" uniqKey="Kim S" first="Seong-Jun" last="Kim">Seong-Jun Kim</name><name sortKey="Kim, Seung Il" sort="Kim, Seung Il" uniqKey="Kim S" first="Seung Il" last="Kim">Seung Il Kim</name><name sortKey="Ku, Keun Bon" sort="Ku, Keun Bon" uniqKey="Ku K" first="Keun Bon" last="Ku">Keun Bon Ku</name><name sortKey="Lee, Chang Seop" sort="Lee, Chang Seop" uniqKey="Lee C" first="Chang-Seop" last="Lee">Chang-Seop Lee</name><name sortKey="Lee, Geonhee" sort="Lee, Geonhee" uniqKey="Lee G" first="Geonhee" last="Lee">Geonhee Lee</name><name sortKey="Lee, Jeong O" sort="Lee, Jeong O" uniqKey="Lee J" first="Jeong-O" last="Lee">Jeong-O Lee</name><name sortKey="Park, Daeui" sort="Park, Daeui" uniqKey="Park D" first="Daeui" last="Park">Daeui Park</name><name sortKey="Park, Edmond Changkyun" sort="Park, Edmond Changkyun" uniqKey="Park E" first="Edmond Changkyun" last="Park">Edmond Changkyun Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/2020/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000340 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/2020/Analysis/biblio.hfd -nk 000340 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= 2020
|étape= Analysis
|type= RBID
|clé= pubmed:32293168
|texte= Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/2020/Analysis/RBID.i -Sk "pubmed:32293168" \
| HfdSelect -Kh $EXPLOR_AREA/Data/2020/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |